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Fresh frozen plasma is ineffective for reversal of coagulation factor dilution in an in vitro model

C. Jámbor1,2, V. Lesch1, T. W. Schnider1,3, G. Kreienbühl1, W. Korte2,3
1Institute for Anaesthesiology and 2Institute for Clinical Chemistry and Hematology, Kantonsspital St. Gallen, Switzerland; 3University of Bern, Switzerland
[Applied Cardiopulmonary Pathophysiology 13: 130-133, 2009]


As fresh frozen plasma (FFP) loses coagulation factor activity during the production process, FFP therapy may not be feasible to completely replenish coagulation factor concentrations in a clinical setting. The aim of this study was to describe the results of early replacement coagulation factors with FFP in a simplified and idealized in vitro model of dilution. Six ml of whole blood drawn from healthy volunteers was first diluted. Imitating blood loss and replacement with FFP thereafter, 20%, 40%, 60% and 80% of the diluted whole blood was removed and replaced with FFP. Levels of coagulation factors were analyzed with standard assays. FFP did not result in replenishing of coagulation factors. Rather, a trend to a further decrease of most coagulation factors was seen. In this in vitro study, a modelled, limited dilution was stabilized but not reversed by replacing (imitated) blood loss with FFP. As continued coagulation factor consumption and additional dilution due to volume replacement occur in the clinical setting, our data suggest that the use of coagulation factor concentrates seem necessary to replenish coagulation factor activities.

Keywords: coagulation factors, in vitro dilution, FFP, transfusion

Address for corresponding:
Csilla Jámbor, M.D., Clinic for Anesthesiology, University of Munich, Max-Lebsche-Platz 32, 81377 Munich, Germany, Öffnet ein Fenster zum Versenden einer

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